MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_NextPart_01C31BFE.53AB1530" This document is a Web archive file. If you are seeing this message, this means your browser or editor doesn't support Web archive files. For more information on the Web archive format, go to http://officeupdate.microsoft.com/office/webarchive.htm ------=_NextPart_01C31BFE.53AB1530 Content-Location: file:///C:/D06BA0B6/selected6.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" 6. Az öröklődés alapjai

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Biológia II.
6. Az öröklődés alapjai
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Az öröklődés alapjai
6. Az öröklődé= ;s alapjai
nGENETIKA
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nAz örökítő anyag szerveződésével és funkciój&= aacute;val, az élőlények tulajdonságinak átöröklődésével foglalkozó tudomány.
= n
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Bár az öröklődés mikéntje és szabályszerűségei évezredek óta foglalkoztatják az emberiséget- amint ezt a mintegy 5000 éves mezopotámiai hieroglifák és a Bibliában leírt történetek is tanúsítják- genetikáról csak Mendel munkásságától beszélhetünk.
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Az öröklődés alapjai
A modern genetika ágai
<= span class=3DBB style=3D'position:absolute;left:-4.72%;top:.39em'>nmende= li (klasszikus) genetika
ntulajdonságok átörökítése a következő nemzedékre
n
<= span class=3DBB style=3D'position:absolute;left:-4.53%;top:.39em'>nmolek= uláris genetika --->genomika
naz öröklődés biokémiai háttere
n
<= span class=3DBB style=3D'position:absolute;left:-4.72%;top:.39em'>npopul= ációgenetika
npopulációk genetikai összetétele
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Genetikai alapfogalmak I.
nGén: a DNS molekula egy szakasza, amely egy tulajdonság megha= tározásáért felelős.
nLókusz: a kr. azon szakasza amin a gén elhelyezkedi= k
nGenotípus: egy élőlény génkészlete egy vagy több gént illetően.
nFenotípus: <= span style=3D'font-size:63%'>a génkészlet működésének eredményeképpen <= /span>kialakuló tulajdonságok összessége.
nAllélok: egy gén alternatív változatai= .
nVad allél: <= span style=3D'font-size:63%'>egy adott génnek a természetben leggyakrabban el= 37;forduló változata.
nLetális allél: egy létfontosságú gén olyan változata, amel= y életképtelenséget, vagy ivarérett kor előtti elhalálozást okoz.
A gén az öröklődés egysége. Egy fehérjemolekula kódolásán keresztül egy tulajdonságért felelős.
Allél: szőrszín esetén fekete, barna, albínó
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Genetikai alapfogalmak II.
nHomoz= igóta: a kr. pár azonos helyein azonos allélok.
<= span class=3DBB style=3D'position:absolute;left:-3.87%;top:.39em'>nHeter= ozigóta: a kr. pár azonos helyein különböző allélok.
<= span class=3DBB style=3D'position:absolute;left:-4.22%;top:.39em'>nHemiz= igóta: csak egyetlen kópiában hordozz= a
nDomin= áns allél:  olyan génváltozat, amely fenotípusosan mindig megjelenik (heterozigótákban is). nReces= szív allél: olyan génváltoza= t, amely fenotípusosan csak homo- és hemizigótákban manifesztálódik. = ;
n
n Jelölések:  A, a allélek
n Lehetséges genotípusok: AA<= span style=3D'font-size:75%'>, Aa, aa
Hemizigót= a pl. az X kromoszóma génjeire egy férfi
Fenotípus= os megjelenés szempontjából beszélünk domináns és recesszív allélekről<= br>
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Mendel kísérletei
<= span style=3D'font-size:88%'>nTudományos kísérletezés módszere
n- alkalmas objektum választása
n- körültekintő kísérlettervezés
= n- sok adat gyűjtése
= n- matematikai értékelés <= /span>
n- következtetések kísérletes ellenőrzése
nA kerti borsó (Pisum sativum)
= n- sok változat
= n- önmegtermékenyítő &eacut= e;s könnyen keresztezhető
n- kis helyigény, rövid tenyészidő
Mendel kort&aacu= te;rsaival ellentétben az egyes tulajdonságok öröklődését vizsgálta, egyszerre csak= egy tulajdonságra koncentrálva. Több generáci&oacut= e;n keresztül figyelemmel kísérte az utódokat.  Eredményeit matematikai (statisztikai) módszerekkel.
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Vizsg= ált tulajdonságok
nvirágszín b= íbor - fehér
nborsószem alakja kerek - szögletes
nsziklevél színe = sárga - zöld
nhüvely alakja felfújt - szemre simuló
nhüvely színe zöld - sárga
nvirágok helyzete axiális - terminális
nszárhossz hosszú - rövid
= n
nTiszta vonal : utódok tulajdonságai megegyeznek a szülőkével
nHibrid: két tiszta vonalú keresztezéséből származó egyed
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K&oum= l;vetkeztetések
nUniformitás el= ve: az első utódnemzedék egye= dei egyformák (feno= típusosan és genotípusukat tekintve) n Szegregáció (hasadás) elve: a második utódnemzedékben az eredeti szülői tulajdonságok megjelennek.
n
nMagyarázat:
n Az ivarsejtekben tulajdonságokat hordozó faktorok vannak (gének), egyesülésükkor az egyedbe 2-2 kerül. n A heterozigóta egyedek egyforma arány= ban termelnek mindkét allélt hordozó ivarsejteket.
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Teszt= -keresztezés
=
nDomináns fenotípusú egyedet recesszív egyeddel keresztezve egyforma (heterozigóta) egyedeket kapunk, ha  a szülő homozigó= ta volt, ha heterozigóta, akkor fele-fele arányban kapunk domináns és recesszív fenotípusú utódokat.
= Aa
= Aa
= A
= Aa
= Aa
= A
= AA
= a
= a
= aa
= aa
= aa
= a
= Aa
= Aa
= A
= Aa
= a
= a
= aa
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Domi= náns - recesszív öröklésmenet
<= span style=3D'font-size:88%'>nRecesszív rendellenességek:
nfenilketanuria, cisztikus fibrózis, albinizmus
nDomináns rendellenességek:
nakondroplázia (törpeség), polydactylia (hatujjúság),
nbrachidactylia (rövidujjúság), Huntington-kór
n(vitustánc)
nEgyéb tulajdonságok:
nRh+ = > Rh-, bar= na szem > kék szem,
nlenőtt fülcimpa > nem lenőtt
Cisztikus fibr&o= acute;zis: genetikai eredetű anyagcserebetegség, tüdőben túlzott mukózus váladéktermeléssel.
Fenilketanuria:<= span style=3D'mso-spacerun:yes'>  Phe->Tyr enzime kiesik, fenol-származékok felszaporodnak. Nem kezelt gyermeken agykárosodás, szellemi visszamaradottság.

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Nemhez kötött öröklődés
nKromoszómák:
<= span style=3D'font-size:75%'>nautoszóm&aa= cute;k (emberben 22 pár)
<= span style=3D'font-size:75%'>nivari kromoszómák (emlősökben XX ♀, XY ♂)=
= XY
= XY
= Y
= XX
= XX
= X
= XY
= X
= X
= XX
A nem meghatározás
Emlősö= k, muslica női a homogamétás nem
Lepkék, m= adarak: hím a homogamétás nem
Elméletil= eg tehát 50-50%-ban születnek fiúk és lányo= k. Valóságban több fiú születik, de a fiúknak nagyobb a halálozásuk.
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Nemhez kötött öröklődés
=
=
=
Anya
=
=
=
Apa
=
=
=
Anya
=
=
=
Apa
=
=
=
X= w+Xw+
=
=
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XwY
=
=
=
Hím gaméták
=
=
=
Hím gaméták
=
=
=
XwXw 
=
=
=
Xw+Y<= b>
=
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Xw
=
=
=
Y
=
=
=
Xw+
=
=
=
Y
=
=
=
P
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=
=
X= w+
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X= w+Xw
=
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Xw+Y
=
=
=
X= w+Xw
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=
=
Xw= Y<= b>
=
=
=
Xw 
=
=
=
N= ői gaméták
=
=
=
N= ői gaméták
=
=
=
F1
=
=
=
♀
=
=
=
♂
=
=
=
♀
=
=
=
♂
Xw+: piros szemszín allélt tartalmaz&oa= cute; X kromoszóma
A w+ allél dominán= s w felett.
Reciprok keresztezés
T. H. Morgan 1910
Dros= ophila melanogaster
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Content-Type: text/html; charset="us-ascii" 6. Az öröklődés alapjai
Példák ivari kromoszómákhoz kötött génekre
n  X
<= span style=3D'font-size:88%'>nvérzé= ;kenység (hemofília)
<= span style=3D'font-size:88%'>nvörös-z&= ouml;ld színtévesztés
<= span style=3D'font-size:88%'>nD-vitamin reziszte= ns angolkór
n
n Y
<= span style=3D'font-size:88%'>nfülcimpa szőrösség
n
------=_NextPart_01C31BFE.53AB1530 Content-Location: file:///C:/D06BA0B6/selected6_elemei/slide0021.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" 6. Az öröklődés alapjai
Inter= medier öröklődés
npiros és fehér virágú = csodatölcsérek keresztezése
n(Carl Correns 1900)
n
nAz F<= /span>1<= /span> nemzedék egyik színe az egyik szülőre sem hasonlít.
<= span style=3D'font-size:63%;visibility:hidden'>n&#= 13;
nköztes (intermedier)
n        = ;            &n= bsp;      öröklődés
nF2<= /span> nemzedékben a fenotípusok megoszlása
= n   1 : 2 : 1
n
nA genotípus fenotípusosan megjelenik.
=
=
=
P
=
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RR<= /b>
=
=
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rr<= /b>
=
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=
X
=
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F2
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Rr<= /b>
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Önmegtermékeny&iac= ute;tés
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F1 =
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1/4 = RR
=
=
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1/4 = Rr
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1/4 = Rr
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1/4 = rr
Gyakran a domina= ncia nem teljes.
C. Correns a Men= deli eredmények egyik újrafelfedezője.
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Az öröklődés alapjai
<= span style=3D'font-size:75%'>nEgy gén t&o= uml;bb allélja is domináns
nHeterozigót= ákban az allélok egymás mellett, egymástól függetlenül megnyilvánulnak
<= span style=3D'font-size:75%'>nPl. ABO vércsoportrendszer
n
nA, B: antigént kódoló allélek
n0: nem kódol antigént
n
n6 genotípus változat
n4 fenotípus
n
nVéradás olyan csoportnak
n lehetséges, amelyik nem
n támadja az adott vér
n antigénjét/antigénjeit.
Kodom= inancia
Feno= típus
A
B
AB<= /b>
Geno= típus
= I
A=
= I
A=
= I
A=
= I
O=
= I
B=
= I
B=
= I
B=
= I
O=
= I
A=
= I
B=
= I
O=
= I
O=
Anti= gén
A
A
B
B
A,<= /b> B
-
O
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Az öröklődés alapjai
Kapcs= oltság
nKét gén együtt öröklődik – egy kr-n nF1 gamét&aa= cute;i csak kétfé= ;lék = nha a gének közel vannak egymá= ;shoz = a kr-n
Mikroszkó= pos vizsgálatok alapján már 1910-ben ismert volt az meiózis folyamata. Ekkor már egyre biztosabban tuddtá= ;k, hogy a gének a kromoszómákon helyezkednek el.=
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Rekom= bináció (Crossing over)
<= span style=3D'font-size:88%'>nKis mennyiségben
nkeletkeznek kevert
nivarsejtek a meiózis
nsorán végbement
ncrossing over
n(átkereszteződés)
nmiatt .
nCO valószínűsége ~
ngének távolsága
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Menny= iség jellegek
nmértékegységgel kifejezhet= 37;, folytonos jellegű tulajdonságok
<= span style=3D'font-size:88%'>nPl. tojásho= zam, tejhozam, testmagasság,
nnagy elemszámú vizsgálat esetén az értékek norm&aa= cute;l eloszlást mutatnak
Figure: 10.17b

Caption:<= br>
(b)The hypothesis illustrated here is that flower color is controlled by a single gene with= two alleles, symbolized R and r. Heterozygotes have an intermed= iate phenotype because of a phenomenon known as incomplete dominance.


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Dohánynövény magassága
       <= /span>   P AABB X aabb
180 cm 60 cm
            F1        = ;  AaBb X AaBb
       <= /span>AABB        AABb        AaBB        AaBb
       AAB= b         AAbb        AaBb         Aabb
       AaB= B         AaBb         aaBB        = ;  aaBb
       AaB= b        = ;  Aabb        = ;  aaBb        = ;  aabb
AB
Ab
aB
ab
F2 = ;
AB        = ;    Ab        = ;      aB        = ;     ab
Dohányn&o= uml;vény magassága
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Mut&a= acute;ciók
nMutáció: Az örökítő anyagban létrejövő, öröklődő változás.
n
nSejttípus szerint
= n- testi sejti ® változás egyeden belül
n- ivarsejti   ® utódban megjelenhet =
n
nKiváltó ok alapján
n- spontán    mutációs ráta
= n- indukált (mesterségesen kiváltott – mutagének )
n
nTípusai
n- génmutáció
n- kromoszóma-mutáció
Testi: utó= ;dsejtek örökítik a változást, de csak abban a szervezetben. Utódokba nem kerül.
ivarsejti: a mutációt hordozó sejtekből utódok származnak.

Természet= es mutációs ráta: 10-4 – 10-10. 100 000 ivarsejtenként mutál egy gén.
Mutagének= :  mutációt serkent&#= 337; anyagok, sugárzások
Pl.  salétromsav, hidrog&eacut= e;n szulfitok, UV sugárzás, gamma sugárzás (radioaktív), röntgensugárzás
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G&eac= ute;nmutációk
-ACGG= GT-
-TGCC= CA-
<= span style=3D'font-size:88%'>nnéma <= /span>
núj triplett ua. AS vagy intronban
<= span style=3D'font-size:88%'>ncsendes
nAS csere fenotípusváltozás nélkül
<= span style=3D'font-size:88%'>ntéveszt&eac= ute;ses
nmás AS ®  funkcióvesztés
<= span style=3D'font-size:88%'>nértelmetlen=
nAS ® STOP
<= span style=3D'font-size:88%'>nlánchosszab= bító
nSTOP® AS
<= span style=3D'font-size:88%'>nframeshift –olvasási keret elcsúszás
frameshift: kiesik/beépül egy bp
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Kromoszóma-mutációk
1. Számbel= i
nFajra jellemző kr. alapkészlet (n) kétszerese
nhelyett több vagy kevesebb kerül az utódba.
n
n
n
n
<= span style=3D'font-size:94%'>nPoliploidia = ;
naz alapszám 2-nél nagyobb egész számú többszöröse
<= span style=3D'font-size:94%'>nAneuploidia = ;
nnem a teljes genomból van több, csak bizonyos
nkromoszómá(k)ból
2n       8n<= /b>
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Aneup= loidia
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<= span class=3DBB style=3D'position:absolute;left:-4.86%;top:.39em'>ntrisz= ómia –egy kr. -ból 3 van
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•21 Down-, 13 Patau-, 18 Edwards-szindróma
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•XXY, XYY, X0
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<= span class=3DBB style=3D'position:absolute;left:-4.2%;top:.39em'>n1/2000 születés
<= span class=3DBB style=3D'position:absolute;left:-4.2%;top:.39em'>nmajdne= m mindig meddő
<= span style=3D'visibility:hidden'>n
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<= span class=3DBB style=3D'position:absolute;left:-4.2%;top:.39em'>nnorm&a= acute;lis szaporodóképesség
<= span class=3DBB style=3D'position:absolute;left:-4.2%;top:.39em'>nnagyobb bűnözési hajlam (?)
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Metan&= #337; (XXX)
Szuper= férfi (XYY)
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Kromoszóma-mut&aac= ute;ciók
2. Szerkezeti
deléci&= oacute;
inverzió= ;
dupliká= ció
transzlok&aacu= te;ció
deléci&oa= cute;: macskasírás szindróma
inverzió = ált. spontán abortuszt okoz
Down transzlokációs változata
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Az öröklődés alapjai
Bepillantás a molekuláris genet= ikába
n1944 Avery et al.
n        = ;    DNS az örökítőanyag
n
n1953 Watson & Crick
= n        = ;          a DNS szerkezete
= n
n1958 Meselson & Stahl
n  szemikonzervatív DNS replikáció
n
n1966 Nirenberg & Khorana= 3;
n        genetikai kód megfejtése
Médi&aacu= te;ban, közéletben sokszor lehet hallani különböző molekuláris biológiai eljárásokról; génszekvenálásról, génklónozásról, klónozásr&oacut= e;l PCR-ről.
A molekulá= ;ris genetika az örökítő anyag biokémiájával foglalkozik. A DNS felépítéséről, funkciójáról a nukleinsavakról szóló fejezetben ill.  nukleinsavak bioszintézise kapcsán említettük.
1944 Avery DNS az örökítőanyag
1966 Nirenberg, = Khorana a genetikai kód megfejtése
Használna= k in vitro fehérjeszintézist.
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Az öröklődés alapjai
T&eac= ute;rképezés
nKromoszó= ma térképezés
n- gének elhelyezkedése a
nkr-n
n- keresztezési kísérletek
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n- fizikai térkép
n- gének távolsága
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<= span style=3D'font-size:75%'>nSzekvená= lás
n- bázissorrend meghat= ározása
A kromoszó= ;ma térképezés: gének elhelyezkedése a kromoszómán egymáshoz képest.  (XX. sz. eleje Morgan tanítványa Alfred Sturtlevant -genetikai térkép –fizikai tulajdonságok alapján.
50’s finom= abb térképek)
Ahhoz, hogy egy = gén működését vizsgáljuk nem elég hogy tudjuk hol van, de a kísérletekhez megfelelő mennyiségben tisztán ki kell vonni. Ha ezt tisztítással szeretnénk elérni pl. egy emberi gén esetén, akkor több kg emberi DNS-ből kellene kiindulni ez azonban lehetetlen.
A génk= lónozás olyan eljárás aminek segítségével a gén pontos másolatait elkészíthetjük, ezáltal válik lehetővé  a DNS-darab szükséges mennyiségben való előállítása.  Ami vizsgálatokon kívül a szekvenáláshoz is szükséges= .

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Az öröklődés alapjai
G&eacu= te;nklónozás
ngenetikailag egyforma DNS darabok előállítása&= #13;
<= span style=3D'font-size:63%;visibility:hidden'>n&#= 13;
n1. DNS bejuttatása egy baktériumba
n- plazmid segítségével
n
n2. Baktérium elszaporítása
n
n3. DNS kinyerés
Klón: tel= jesen azonos, eredetileg: gally
Nem új ke= letű dolog a növények körében általános és elterjedt (inda, gyökérsarj, dugvány… stb.)
A génklónozást –hasonlóan a joghurtkészítéshez- az ember nem maga végzi h= anem kiadja apró béreseknek, akik ellátás fejében elvégzik.
A baktéri= umoknak  általában egy db gyűrű alakú DNS-ből áll a genetikai állományuk –előző félév. Ezen kívül lehetnek bennük kiegészítő genetikai elemek: az ún. plazmidok, amelyek általában antibiotikum rezisztenciát kódolnak. Ezeket a kis, cirkuláris DNS darabokat használják fel.
A restrikci&oacu= te;s endonukleázok olyan DNS hasító enzimek, amelyek meghatározott szekvenciákat ismernek fel és mindkét szálat elvágják, mégpedig ferdén -> ragadós vég keletkezik. Ha a klónozni kívánt DNS szakasz is ilyen végekkel rendelkezik, akkor a plazmiddal összekapcsolódnak.
Ezt az át= szerkesztett plazmidot pl. egy vírus segítségével bejuttatják egy baciba. Amit ezután antibiotikumos táptalajon elszaporítanak. Így csak azok maradnak meg amelyek hordozzák a plazmidot. Ezekből tisztítá= ssal kinyerhető a kívánt DNS a megfelelő mennyiségben.
Ezzel a mó= ;dszerrel visznek be egy élőlénybe genetikai információt, pl. hasonlóan lehet rábeszélni a feketepenészt, hogy citromsavat termelj= en. (Eukariban nem plazmid, hanem mesterséges kromoszóma) Biztosítékként úgy csinálják a plazmidot, hogy a hasítás is rezisztenciagénben legy= en, hogy a manipulált jószág érzékeny legy= en vmire –nehogy elszabaduljon.
Ez a móds= zer elvileg alkalmas gének emberi sejtekbe való bejuttatás&aacut= e;ra is.  Kísérletek több kevesebb sikerrel. A manipulált, majd visszajuttatott se= jtek általában rövidebb életűek.
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Az öröklődés alapjai
3D"Szövegdoboz:
A
SANGER-módszer
1. ssDNS
2. DNS szintézis
4 kémcsőben
• dideoxi-NTPok
ddATP
ddCTP
ddGTP
ddTTP
• adott nukleotidnál megszakad a szintézis
3. Gélel= ektroforézis
4. előhívás <= /div>
5. leolvasás
1975 Friedrich S= anger és mtsi egy módszert dolgoztak ki a DNS bázissorrendjének meghatározására (1980 Nobel)
A módszer lényege:
1. egyszá= lú DNS darabot hoznak létre –kiindulás
2. arről, m= int templátról DNS-t szintetizálnak egy ismert primerből kiindulva.
3. a szint&eacut= e;zist 4 kémcsőben végzik, mindegyik más módosított NTP-t tartalmaz:
dideoxi-ATP,ddCT= P,…
Ezek megszakítják  a DNS-szintézist. Első kémcsőben minden olyan darab szintetizálódik, ami A-re végződik…
A kapott mint&aa= cute;kat gélelektroforézissel megfuttatják
Leolvashat&oacut= e; a nukleotid-sorrend.

Automata szekven= átor.
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Az öröklődés alapjai
= DNS

fingerprint=
(ujjlenyomat)
Szinte egyedi mintázat
• DNS hasítás <= span style=3D'position:absolute;top:52.0%;left:78.65%;width:20.59%;height:4.0%'= >restrikciós enzimmel
•szétválas= ztás (gélelektroforézis)
•
Nem minden esetb= en szükséges a teljes szekvencia. A DNS ujjlenyomat egyedi, mint= az ujjlenyomat. DNS-t restrikciós enzimmel nem túl kicsi darab= okra hasítják. A fragmentumokat elektroforézissel szétválasztják.
Az így ka= pott mintázat egyedi, sőt öröklődik, közeli rokonoknak hasonló. Így felhasználható kriminalisztikai azonosításra és apaságvizsgálatra is. Apaságot nemcsak kizár,= de bizonyít is!!
MELYIK A GYILKOS= ?

Ennek az eljárásnak az előfeltétele, hogy gyorsan fel lehessen szaporítani a DNS-mintát, hiszen egy emberből csak kevés mintát vehetünk, nem is beszélve arról hogy a tett színhelyén mennyit találunk= .

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Az öröklődés alapjai
PCR<= /span>
n     polimeráz láncreakció
nkétszálú DNS láncainak= szétválasztása
nprimerek hozzáadása
nDNS-polimeráz komplementer DNS szálakat hoz szintetizál =
nújra szétválasztásR= 30;
A módszer= ami ezt megvalósítja  = PCR (Polimerase Chain Reaction).
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quoted-printable Content-Type: text/html; charset="us-ascii" 6. Az öröklődés alapjai
A centrális dogma, kivételek
nDNS ® RNS ® fehérje
<= span style=3D'font-size:50%;visibility:hidden'>n= 3;
<= span style=3D'font-size:88%'>nreverz transzkripció
nDNS függő RNS polimeráz
n RNS ® DNS
nretrovírusok szaporodása
n
<= span style=3D'font-size:88%'>ncDNS  (kópia) =
nmRNS-ről készült DNS másolat
n
1970 H. Termin &= amp; D. Baltimore  RNS függ!= 7; DNS polimeráz (reverz transzkriptáz)
Alkalmazá= s
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‹#›
Bár az öröklődés mikéntje és szabályszerűségei évezredek óta foglalkoztatják az emberiséget- amint ezt a mintegy 5000 éves mezopotámiai hieroglifák és a Bibli&aacut= e;ban leírt történetek is tanúsítják- genetikáról csak Mendel munkásságától beszélhetünk.
A gén az öröklődés egysége. Egy fehérjemolekula kódolásán keresztül egy tulajdonság&eacut= e;rt felelős.
Allél: szőrszín esetén fekete, barna, albínó
Hemizigóta pl. az X kromoszóma génjeire egy férfi
Fenotípusos megjelenés szempontjából beszélünk domináns és recesszív all&eacut= e;lekről
Mendel kortársaival ellentétben az egyes tulajdonságok öröklődését vizsgálta, egyszerre csak = egy tulajdonságra koncentrálva. Több generáció= ;n keresztül figyelemmel kísérte az utódokat.  Eredményeit matematikai (statisztikai) módszerekkel.
Cisztikus fibrózis: genet= ikai eredetű anyagcserebetegség, tüdőben túlzott m= ukózus váladéktermeléssel. Fenilketanuria:  Phe->Tyr enzime kiesik, fenol-származékok felszaporodnak. Nem kezelt gyermeken agykárosodás, szellemi visszamaradottság.
 
Emlősök, muslica női a homogamétás nem&#= 13;
Lepkék, madarak: hím a homogamétás nem= 3;
Elméletileg tehát 50-50%-ban születnek fiúk és lányok. Valóságban több fiú sz&= uuml;letik, de a fiúknak nagyobb a halálozásuk.
Gyakran a dominancia nem teljes.
C. Correns a Mendeli eredmények egyik újrafelfedező= ;je.
Mikroszkópos vizsgá= ;latok alapján már 1910-ben ismert volt az meiózis folyamata. Ekkor már egyre biztosabban tuddták, hogy a gének a k= romoszómákon helyezkednek el.
Figu= re: 10.17b
= ;
Capt= ion:
(b)The hy= pothesis illustrated here is that flower color is controlled by a single gene wit= h two alleles, symbolized R and r. Heterozygotes= have an intermediate phenotype because of a phenomenon known as incomplete dominance.
= ;
Dohánynövény magassága
Testi: utódsejtek örökítik a változást, de csak abban a szervezetben. Utódokba nem kerül.
ivarsejti: a mutációt hordozó sejtekből utódok származnak.
Természetes mutációs ráta: 10-4=  – 10-10. 100 000 ivarsejtenként mutál egy gén.
Mutagének:  mutációt serkentő anyagok, sugárzások
Pl.  salétromsav, hidrogén szulfitok, UV sugárzás, gamma sugárzás (radioaktív), röntgensugárz&aacu= te;s
frameshift: kiesik/beép&u= uml;l egy bp
deléció: macskasírás szindróma = ;
inverzió ált. spontán abortuszt okoz
Down transzlokációs változata
Médiában, közé= letben sokszor lehet hallani különböző molekuláris biológiai eljárásokról; génszekvenálásról, génklónozásról, klónozásró= ;l PCR-ről.
A molekuláris genetika az örökítő anyag biokémiájával foglalkozik. A DNS felépítéséről, funkciójáról a nukleinsavakról szól&oac= ute; fejezetben ill.  nukleinsavak bioszintézise kapcsán említettük.
1944 Avery DNS az örökítőanyag
1966 Nirenberg, Khorana a genetikai kód megfejtése
Használnak in vitro fehérjeszintézist.
A kromoszóma térk&eacut= e;pezés: gének elhelyezkedése a kromoszómán egymáshoz képest.  (XX. sz. eleje Morgan tanítványa Alfred Sturtlevant -genetikai térkép –fizikai tulajdonságok alapján.
50’s finomabb térképek)
Ahhoz, hogy egy gén működését vizsgáljuk nem elég hogy tudjuk hol van, de a kísérletekhez megfelelő mennyiségben tisztán ki kell vonni. Ha ezt tisztítással szeretnénk elérni pl. egy emberi gén esetén, akkor több kg emberi DNS-ből kellene kiindulni ez azonban lehetetlen.
A génklónozás olyan eljárás amin= ek segítségével a gén pontos másolatait el= készíthetjük, ezáltal válik lehetővé  a DNS-darab szükséges mennyiségben való előállítása.  Ami vizsgálatokon kívül a szekvenáláshoz is szükséges.
Klón: teljesen azonos, eredetileg: gally
Nem új keletű dolog a növények körében általános és elterjedt (inda, gyökérsarj, dugvány… stb.) <= /div>
A génklónozást –hasonlóan a joghurtkészítéshez- az ember nem maga végzi hanem kiadja apró béreseknek, akik ellátás fejében elvégzik.
A baktériumoknak  általában egy db gyűrű alakú DNS-ből áll a genetikai állományuk –előző félév. Ezen kívül lehetnek bennük kiegészítő genet= ikai elemek: az ún. plazmidok, amelyek általában antibioti= kum rezisztenciát kódolnak. Ezeket a kis, cirkuláris DNS darabokat használják fel.
A restrikciós endonukleázok olyan DNS hasító enzimek, amelyek meghatározott szekvenciákat ismernek fel és mindkét szálat elvágják, mégpedig ferdén -> ragadós vég keletkezik. Ha a klónozni kívánt DNS szaka= sz is ilyen végekkel rendelkezik, akkor a plazmiddal összekapcsolódnak.
Ezt az átszerkesztett plazmidot p= l. egy vírus segítségével bejuttatják egy baci= ba. Amit ezután antibiotikumos táptalajon elszaporítanak. Így csak azok maradnak meg amelyek hordozzák a plazmidot. Ezekből tisztítással kinyerhető a kív&aacut= e;nt DNS a megfelelő mennyiségben.
Ezzel a módszerrel visznek be egy élőlénybe genetikai információt, pl. hasonlóan lehet rábeszélni a feketepenészt, ho= gy citromsavat termeljen. (Eukariban nem plazmi= d, hanem mesterséges kromoszóma) Biztosítékként úgy csinálják a plazmidot, hogy a hasítás is rezisztenciagénben legye= n, hogy a manipulált jószág érzékeny legyen vmire –nehogy elszabaduljon.
Ez a módszer elvileg alkalmas gének emberi sejtekbe való bejuttatására is.  Kísérletek több kevesebb sikerrel. A manipulált, majd visszajuttatott sejtek általában rövidebb életűek.
1975 Friedrich Sanger és = mtsi egy módszert dolgoztak ki a DNS bázissorrendjének meghatározására (1980 Nobel)
A módszer lényege:
1. egyszálú DNS darabot hoznak létre –kiindulás
2. arről, mint templátról DNS-t szintetizál= nak egy ismert primerből kiindulva.
3. a szintézist 4 kémcsőben végzik, mindegyik más módosított NTP-t tartalmaz:
dideoxi-ATP,ddCTP,…
Ezek megszakítják  a DNS-szintézist. Első kémcsőben minden olyan darab = szintetizálódik, ami A-re végződik…
A kapott mintákat gélelektroforézissel megfuttatják
Leolvasható a nukleotid-sorrend.
Automata szekvenátor.
Nem minden esetben szüks&ea= cute;ges a teljes szekvencia. A DNS ujjlenyomat egyedi, mint az ujjlenyomat. DNS-t restrikciós enzimmel nem túl kicsi darabokra hasítják. A fragmentumokat elektroforézissel szétválasztják. Az így kapott mintá= zat egyedi, sőt öröklődik, közeli rokonoknak hasonló. Így felhasználható kriminalisztikai azonosításra és apaságvizsgálatra is. Apaságot nemcsak kizár, de bizonyít is!!
MELYIK A GYILKOS?
Ennek az eljárásnak az előfeltétele, hogy gyorsan fel lehessen szaporítani a DNS-mintát, hiszen egy emberb!= 7;l csak kevés mintát vehetünk, nem is beszélve arról hogy a tett színhelyén mennyit találunk.
A módszer ami ezt megvalósítja  P= CR (Polimerase Chain Reaction).
1970 H. Termin & D. Baltimor= e  RNS függő DNS polimeráz (reverz transzkriptáz)
Alkalmazás
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MakeNotesVis() ) return runAnimations =3D _InitAnimations(); if( IsWin("PPTSld") ) parent.SldUpdated(GetSldId()) g_origSz=3DparseInt(SlideObj.style.fontSize) g_origH=3Dsld.style.posHeight g_origW=3Dsld.style.posWidth g_scaleHyperlinks=3D(document.all.tags("AREA").length>0) if( g_scaleHyperlinks ) InitHLinkArray() if( g_scaleInFrame||(IsWin("PPTSld") && parent.IsFullScrMode() ) ) document.body.scroll=3D"no" _RSW() if( IsWin("PPTSld") && parent.IsFullScrMode() ) FullScrInit(); =09 MakeSldVis(); ChkAutoAdv() if( runAnimations ) { if( document.all("NSPlay") ) document.all("NSPlay").autoStart =3D false; if( sld.filters && sld.filters.revealtrans ) setTimeout( "document.body.start()", sld.filters.revealtrans.duration * = 1000 ); else document.body.start(); } } function MakeSldVis() { var fTrans=3Dg_showAnimation && SldHasTrans() if( fTrans ) { if( g_bgSound ) { idx=3Dg_bgSound.indexOf(","); pptSound.src=3Dg_bgSound.substr( 0, idx ); pptSound.loop=3D -(parseInt(g_bgSound.substr(idx+1))); } SlideObj.filters.revealtrans.Apply() } SlideObj.style.visibility=3D"visible" if( fTrans ) SlideObj.filters.revealtrans.Play() } function MakeNotesVis() { if( !IsNts() ) return false SlideObj.style.display=3D"none" nObj =3D document.all.item("NotesObj") parent.SetHasNts(0) if( nObj ) { nObj.style.display=3D"" parent.SetHasNts(1) } return 1 } function ChkAutoAdv() { if(SldHasTrans()) SlideObj.onfilterchange=3DAutoAdv else AutoAdv() } function AutoAdv() { if(!IsWin("PPTSld") || !gUseSldTimings )return var sld=3DGetCurSld() if( (sld.mAdvDelay>0) && !parent.IsFramesMode() ) setTimeout("parent.GoToNextSld()",sld.mAdvDelay) } function GetObj(id) { if(g_supportsPPTHTML) return document.all(id); else return document.getElementById(id); } function SldHasTrans() { return SlideObj.style.getAttribute("filter")!=3D""= } function GetSldId() { return sId=3Dlocation.href.substring(location.href.la= stIndexOf('/')+1) } function HideMenu() { if( frames["PPTSld"] && PPTSld.document.all.item("ctx= tmenu") && PPTSld.ctxtmenu.style.display!=3D"none" ) { PPTSld.ctxtmenu.styl= e.display=3D'none'; return true } return false } function IsWin( name ) { return window.name =3D=3D name } function IsNts() { return IsWin("PPTNts") } function IsSldOrNts() { return( IsWin("PPTSld")||IsWin("PPTNts") ) } function SupportsPPTAnimation() { return( navigator.platform =3D=3D "Win32"= && navigator.appVersion.indexOf("Windows")>0 ) } function SupportsPPTHTML() { var appVer=3Dnavigator.appVersion, msie=3DappVer.indexOf("MSIE "), ver=3D0 if( msie >=3D 0 ) ver=3DparseFloat( appVer.substring( msie+5, appVer.indexOf(";",msie) ) ) else ver=3DparseInt(appVer) return( ver >=3D 4 && msie >=3D 0 ) } function _RSW() { if( !g_supportsPPTHTML || IsNts() || ( !g_scaleInFrame && (!IsWin("PPTSld") || !parent.IsFullScrMode()) ) ) return var padding=3D0; if( IsWin("PPTSld") && parent.IsFramesMode() ) padding=3D6 cltWidth=3Ddocument.body.clientWidth-padding cltHeight=3Ddocument.body.clientHeight-padding factor=3D(1.0*cltWidth)/g_origW if( cltHeight < g_origH*factor ) factor=3D(1.0*cltHeight)/g_origH newSize =3D g_origSz * factor if( newSize < 1 ) newSize=3D1 s=3DSlideObj.style s.fontSize=3DnewSize+"px" s.posWidth=3Dg_origW*factor s.posHeight=3Dg_origH*factor s.posLeft=3D(cltWidth-s.posWidth+padding)/2 s.posTop=3D(cltHeight-s.posHeight+padding)/2 if( g_scaleHyperlinks ) ScaleHyperlinks( factor ) } function _InitAnimations() { animRuntimeInstalled =3D ''+document.body.localTime !=3D 'undefined'; isFullScreen =3D (window.name =3D=3D "PPTSld") && !parent.IsFramesMode(); g_animUseRuntime =3D g_showAnimation && animRuntimeInstalled && !(isFullSc= reen && parent.IsSldVisited()); if( g_animUseRuntime ) { collSeq =3D document.all.tags("seq"); if( collSeq !=3D null ) { for(ii=3D0;ii numSlds ) gSldJumpIdx =3D numSlds; if ( gSldJumpIdx >=3D 0 ) { if ( gSldJumpIdx =3D=3D 0 ) gSldJumpIdx =3D 1; var jumpTo =3D parseInt(gSldJumpIdx); gSldJump =3D 0; gSldJumpIdx =3D ""; win.GoToSld( parent.GetSldList().mList[jumpTo-1].mSldHref ) } } } function _KDH() { if( event.keyCode =3D=3D 8 ) { event.returnValue =3D 0; parent.GoToPrevSld(); } } function DocumentOnClick() { if( IsNts() || parent.HideMenu() ) return; if( ( g_allowAdvOnClick && !parent.IsFramesMode() ) || (event && (event.keyCode=3D=3D32) ) ) parent.GoToNextSld(); } var g_supportsPPTHTML =3D SupportsPPTHTML(), g_scaleInFrame =3D 1, gId=3D""= , g_bgSound=3D"", g_scaleHyperlinks =3D false, g_allowAdvOnClick =3D 1, g_showInBrowser = =3D 0, gLoopCont =3D 0, gUseSldTimings =3D 1; var g_showAnimation =3D g_supportsPPTHTML && SupportsPPTAnimation() && ( (w= indow.name=3D=3D"PPTSld" && !parent.IsFramesMode()) || g_showInBrowser );va= r g_animManager =3D null; var g_animUseRuntime =3D false; var g_animItemsToHide, g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"Vége a diavetítésnek, kattint&aacu= te;sra kilép.", SCREEN_MODE=3D"Frames", gIsEndShow=3D0, NUM_VIS_SLDS= =3D33, SCRIPT_HREF=3D"script.js", FULLSCR_HREF=3D"fullscreen.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var gMainDoc=3Dnew Array(new hrefList("slide0046.htm",1,-1,1),new hrefList(= "slide0002.htm",1,-1,1),new hrefList("slide0004.htm",1,-1,1),new hrefList("= slide0005.htm",1,-1,1),new hrefList("slide0006.htm",1,-1,1),new hrefList("s= lide0007.htm",1,-1,1),new hrefList("slide0008.htm",1,-1,1),new hrefList("sl= ide0010.htm",1,-1,1),new hrefList("slide0013.htm",1,-1,1),new hrefList("sli= de0017.htm",1,-1,1),new hrefList("slide0018.htm",1,-1,1),new hrefList("slid= e0019.htm",1,-1,1),new hrefList("slide0020.htm",1,-1,1),new hrefList("slide= 0021.htm",1,-1,1),new hrefList("slide0022.htm",1,-1,1),new hrefList("slide0= 023.htm",1,-1,1),new hrefList("slide0024.htm",1,-1,1),new hrefList("slide00= 25.htm",1,-1,1),new hrefList("slide0027.htm",1,-1,1),new hrefList("slide002= 9.htm",1,-1,1),new hrefList("slide0030.htm",1,-1,1),new hrefList("slide0031= .htm",1,-1,1),new hrefList("slide0032.htm",1,-1,1),new hrefList("slide0033.= htm",1,-1,1),new hrefList("slide0036.htm",1,-1,1),new hrefList("slide0037.h= tm",1,-1,1),new hrefList("slide0038.htm",1,-1,1),new hrefList("slide0039.ht= m",1,-1,1),new hrefList("slide0040.htm",1,-1,1),new hrefList("slide0041.htm= ",1,-1,1),new hrefList("slide0042.htm",1,-1,1),new hrefList("slide0043.htm"= ,1,-1,1),new hrefList("slide0045.htm",1,-1,1)); function FullScrInit() { g_allowAdvOnClick =3D GetCurSld().mAdvOnClk document.body.style.backgroundColor=3D"black" document.oncontextmenu=3Dparent._CM; document.onkeydown =3D _KDH; document.ondragstart=3DCancel document.onselectstart=3DCancel self.focus() } function Redirect( frmId ) { var str=3Ddocument.location.hash,idx=3Dstr.indexOf('#'), sId=3DGetSldId() if(idx>=3D0) str=3Dstr.substr(1); if( window.name !=3D frmId && ( sId !=3D str) ) { obj =3D GetObj("Main-File") window.location.href=3Dobj.href+"#"+sId return 1 } return 0 } var MHTMLPrefix =3D CalculateMHTMLPrefix(); function CalculateMHTMLPrefix() { if ( document.location.protocol =3D=3D 'mhtml:') { href=3Dnew String(document.location.href) Start=3Dhref.indexOf('!')+1 End=3Dhref.lastIndexOf('/')+1 if (End < Start) return href.substring(0, Start) else return href.substring(0, End) } return ''; } function GetTags(base,tag) { if(g_supportsPPTHTML) return base.all.tags(tag); else return base.getElementsByTagName(tag); } function UpdNtsPane(){ if(frames["PPTNts"]) PPTNts.location.replace( MHTMLP= refix+GetHrefObj( gCurSld ).mNtsHref ) } function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() } function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() } function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() } function SetHasNts( fVal ) { if( gHasNts !=3D fVal ) { gHasNts=3DfVal UpdNavPane() } } function ToggleOtlText() { gOtlTxtExp=3D!gOtlTxtExp UpdOtlPane() } function ToggleOtlPane() { frmset=3Ddocument.all("PPTHorizAdjust") frm=3Ddocument.all("PPTOtl") if( gOtlOpen ) frmset.cols=3D"*,100%" else frmset.cols=3D"25%,*" gOtlOpen=3D!gOtlOpen frm.noResize=3D!frm.noResize UpdOtNavPane() } function ToggleNtsPane() { frmset=3Ddocument.all("PPTVertAdjust") frm=3Ddocument.all("PPTNts") if( gNtsOpen ) frmset.rows=3D"100%,*" else frmset.rows=3D"*,20%" gNtsOpen=3D!gNtsOpen UpdNtsPane() } function ClearMedia() { if (PPTSld.pptSound) PPTSld.pptSound.loop =3D 0; } function FullScreen() { if ( PPTSld.g_animUseRuntime ) PPTSld.document.body.pause(); ClearMedia(); var href =3D ( document.location.protocol =3D=3D 'mhtml:') ? FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else return gCurSld } function GetNumSlds() { if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; PPTSld.location.replace(MHTMLPrefix+obj.mSldHref) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 PPTSld.location.replace(MHTMLPrefix+href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document doc.open() doc.writeln('


' + ENDSHOW_MESG + '

') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); function ImgBtn( oId,bId,w,action ) { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } function TxtBtn( oId,oeId,action,chkState ) { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new TxtBtn( "nb_nts","nb_ntsElem",parent.ToggleNtsPan= e,GetNtsState ) gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","nb_prevBorder",30,parent.GoToP= revSld ) gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","nb_nextBorder",30,parent.GoToN= extSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D new ImgBtn( "nb_voice","nb_voiceBorder",18,parent.= ToggleVNarration ) gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlTxt","nb_otlTxtBorder",23,parent= .ToggleOtlText ) gBtnArr["nb_nts"].m_flagId=3D "notes_flag" gBtnArr["nb_nts"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState var sNext=3D"Következő",sPrev=3D"Előző",sEnd=3D"Bemutat= ó vége",sFont=3D"Arial",sArrow=3D"Nyíl",sFreeform=3D"S= zabadkézi sokszög",sRect=3D"Téglalap",sOval=3D"Ellipszis" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); 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charset="us-ascii" 6. 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